Generator

Part:BBa_K917013

Designed by: Jakub Krakowiak   Group: iGEM12_Edinburgh   (2012-09-24)


pLac promoter plus cytochrome maturation gene cluster ccmA-H

This part consists of the standard registry lac promoter (BBa_J33207) plus Escherichia coli cytochrome maturation gene cluster ccmA-H with native RBS (BBa_K917006).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1237
    Illegal BamHI site found at 4026
    Illegal BamHI site found at 4254
    Illegal BamHI site found at 6598
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 891
    Illegal AgeI site found at 1718
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5305


[edit]
Categories
Parameters
None